Ubiquitination is an important PTM in cell biology, as the ubiquitinated proteasome complex has mediated degradation of 80-85 percent proteins, and directly affects the expression level and function of proteins. MtoZ Biolabs is glad to offer ubiquitination identification and quantification service through high-resolution mass spectrometry analysis. For some low abundance ubiquitinated proteins, we can provide peptide enrichment service before mass spectrometry analysis. To ensure high specificity and efficacy of enrichment of ubiquitinated peptides, we use CST Ubiquitin antibody for enrichment process, and use 2-3 different enzymes for protein digestion to ensure full scan of ubiquitinated peptides.
MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for ubiquitinated proteomics analysis service. To better solve your research problems, we offer an all-inclusive service, including protein purification, digestion, peptide enrichment and separation, LC-MS/MS analysis, MS data analysis and bioinformatics analysis.
